Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT6G11
- UniProt No.
- Q9UBR2
- NCBI Accession No.
- NP_001327
- Alternative names
- Carboxypeptidase LB, Cathepsin B2, Cathepsin IV, Cathepsin P, Cathepsin X, Cathepsin Y, Cathepsin Z, Cathepsin Z preproprotein, Cathepsin Z1, CatX, CTSP, CTSX, CTSZ, Cysteine-type carboxypeptidase, Lysosomal carboxypeptidase B
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human CTSZ (62-303aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of CTSZ in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTSZ antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western blot analysis (WB)
The cell lysates of HeLa (40ug) and SW480 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSZ antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSZ antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : Recombinant Human CTSB
Lane 2. : Recombinant Human CTSD
Lane 3. : Recombinant Human CTSE
Lane 4. : Recombinant Human CTSF
Lane 5. : Recombinant Human CTSH
Lane 6. : Recombinant Human CTSK
Lane 7. : Recombinant Human CTSL
Lane 8. : Recombinant Human CTSS
Lane 9. : Recombinant Human CTSW
Lane 10. : Recombinant Human CTSZ
Lane 1. : Recombinant Human CTSB
Lane 2. : Recombinant Human CTSD
Lane 3. : Recombinant Human CTSE
Lane 4. : Recombinant Human CTSF
Lane 5. : Recombinant Human CTSH
Lane 6. : Recombinant Human CTSK
Lane 7. : Recombinant Human CTSL
Lane 8. : Recombinant Human CTSS
Lane 9. : Recombinant Human CTSW
Lane 10. : Recombinant Human CTSZ
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.