Human Akt1 antibody

Cat No.
ATGA0480

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT6D5
UniProt No.
P31749
NCBI Accession No.
NP_001014432
Alternative names
Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human AKT1 (1-480aa) purified from insect cell.
Isotype
IgG2a kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF, FACS
Usage
The antibody has been tested by Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

ICC/IF analysis of AKT1/3 in A549 cells. The cell was stained with ATGA0480 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKT1/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: NIH3T3 cell lysate
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKT1/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human AKT1
Lane 2.: Recombinant Human AKT2
Lane 3.: Recombinant Human AKT3

Flow cytometry (FACS)

Flow cytometry analysis of AKT1/3 in A549 cells. The cell was stained with ATGA0480 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.