Human TDP-43/TARDBP antibody

Cat No.
ATA0834

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
k1B8
UniProt No.
Q13148
NCBI Accession No.
NP_031401
Alternative names
TAR DNA binding protein, TDP-43, ALS10, TAR DNA-binding protein 43, TDP43

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human TARDBP (1-260aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, IHC, FACS
Usage
The antibody has been tested by ELISA, Western blot, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The cell lysates (20ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TARDBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of TARDBP in HeLa cells. The cell was stained with ATA0834 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Immunohistochemistry (IHC)

Paraffin embedded sections of human prostate cancer tissue were incubated with anti-human TARDBP(1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Paraffin embedded sections of human seminoma tissue were incubated with anti-human TARDBP(1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.