Recombinant human IgG1 Fc protein

Cat No.
ATGP4094

Size

QTY

List Price

10ug

USD 72


20ug

USD 124

50ug

USD 169

100ug

USD 280

250ug

USD 488

500ug

USD 839

1mg

USD 1,398

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
HEK293
Domain
100-330aa
UniProt No.
P01857
NCBI Accession No.
CAC20454.1
Alternative names
immunoglobulin heavy chain constant region gamma 1, IGHG1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE

Product Specification

Molecular Weight
25.9kDa (231aa)
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity
> 90% by SDS-PAGE
Bioactivity
1. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4102). The ED50 range ≤ 1.5 ug/ml.
2. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4116).
3. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4098). The ED50 range ≤ 4 ug/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Biological Activity

Human Fc gamma RIIIA/CD16a (CAT# ATGP4102) is coated at 10 ug/ml (100 ul/well) can bind Human IgG1 Fc. The ED50 range ≤ 1.5 ug/ml.
Human IgG1 Fc is coated at 1ug/ml (100 ul/well) can bind Human Fc gamma RIIA/CD32a (H167) (CAT# ATGP4116) in a Functional ELISA assay.
Human Fc gamma RIIIA/CD16a (CAT# ATGP4098) is coated at 10 ug/ml (100 ul/well) can bind Human IgG1 Fc. The ED50 range ≤ 4 ug/ml.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.