Recombinant human TNF-alpha protein

Cat No.
ATGP3152

Size

QTY

List Price

10ug

USD 80


20ug

USD 140

50ug

USD 235

100ug

USD 390

250ug

USD 920

500ug

USD 1,500

1mg

USD 2,500

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
Baculovirus
Domain
77-233aa
UniProt No.
P01375
NCBI Accession No.
NP_000585.2
Alternative names
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

Product Specification

Molecular Weight
18.1 kDa (163aa)
Concentration
1mg/ml (determined by absorbance at 280nm)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Biological Activity

Human TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.