Recombinant human IL-12 p70 protein

Cat No.
ATGP2843

Size

QTY

List Price

10ug

USD 80


50ug

USD 240

250ug

USD 960

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
Baculovirus
Domain
23-328aa(p40)/23-219aa(p35)
UniProt No.
P29460(p40)/P29459(p35)
NCBI Accession No.
NP_002178(p40)AAK84425(p35)
Alternative names
IL12A, IL12B, CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, Interleukin 12 alpha chain, Interleukin-12 beta chain, NK cell stimulatory factor chain 1, p35, NK cell stimulatory factor chain 2, p40, NKSF1, NKSF2

Product Specification

Molecular Weight
34.6 kDa (p40, 306aa)
23.3 kDa (p35, 203aa)
The heterodimeric IL12 has a predicted Molecular weight of 57.9 kDa (509aa). Molecular weight on SDS-PAGE will appear higher.
Concentration
0.5mg/ml (determined by absorbance at 280nm)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity
> 90% by SDS-PAGE
Bioactivity
The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤1ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Biological Activity

Human IL12 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ≤1 ng/ml.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.