Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-414aa
- UniProt No.
- O75874
- NCBI Accession No.
- NP_005887.2
- Alternative names
- Isocitrate dehydrogenase [NADP] cytoplasmic, IDCD, IDH, IDP, IDPC, PICD
Product Specification
- Molecular Weight
- 48.8 kDa (434aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT,0.1mM PMSF
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 18,000pmol/min/ug. One unit will oxidize 1.0pmole of DL-Isocitrate to D alpha-Ketoglutarate per minute in the presence of beta-NADP at pH7.4 at 37C.
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.