Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 22-153aa
- UniProt No.
- P60568
- NCBI Accession No.
- NP_000577.2
- Alternative names
- IL-2, TCGF, Aldesleukin, T-cell growth factor, Interleukin-2
Product Specification
- Molecular Weight
- 15.4 kDa (133aa) confirmed by MALDI-TOF
- Concentration
- 0.5mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4)
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 range ≤0.65ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Human IL-2 (C145S) stimulates cell proliferation of the CTLL2 mouse cytotoxic T cells. The ED50 range ≤0.65 ng/ml.
Related Publications
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Kim HR, et al. Anti-cancer activity and mechanistic features of a NK cell activating molecule. (Cancer Immunol Immunother. 2009)
Koh J, et al. Susceptibility of CD24(+) ovarian cancer cells to anti-cancer drugs and natural killer cells. 9Biochem Biophys Res Commun. 2012)
Lee HM, et al. A comparative study of the effects of inhibitory cytokines on human natural killer cells and the mechanistic features of transforming growth factor-beta. (Cell Immunol. 2014)
Shin HW, et al. Role of c-Myb in the regulation of natural killer cell activity. (Biochem Biophys Res Commun. 2018)
Lee Y, et al. In vivo Anti-Cancer Effects of Resveratrol Mediated by NK Cell Activation. (J Innate Immun. 2020)
Lee YJ, et al. Resveratrol Activates Natural Killer Cells through Akt- and mTORC2-Mediated c-Myb Upregulation. (Int J Mol Sci. 2020)
Ko E, et al. ADSC secretome constrains NK cell activity by attenuating IL-2-mediated JAK-STAT and AKT signaling pathway via upregulation of CIS and DUSP4. (Stem Cell Res Ther. 2023)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.