Recombinant human TNF-alpha protein

Cat No.
TNF0501

Size

QTY

List Price

20ug

USD 55


50ug

USD 100

100ug

USD 170

250ug

USD 350

500ug

USD 505

1mg

USD 840

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
E.coli
Domain
77-233aa
UniProt No.
P01375
NCBI Accession No.
NP_000585.2
Alternative names
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

Product Specification

Molecular Weight
17.5 kDa (158aa) confirmed by MALDI-TOF
Concentration
1mg/ml (determined by Bradford assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Biological Activity

Human TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Related Publications

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    Cho K, et al. Inhibition of TNF-α-Mediated NF-κB Transcriptional Activity by Dammarane-Type Ginsenosides from Steamed Flower Buds of Panax ginseng in HepG2 and SK-Hep1 Cells. (Biomol Ther (Seoul). 2014)

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    Kim S, et al. Hybrid nutraceutical of 2-ketoglutaric acid in improving inflammatory bowel disease: Role of prebiotics and TAK1 inhibitor. (Biomed Pharmacother. 2024)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.