NKMAX

Product Information

Product Type

Recombinant Protein

Expression System

E.coli

Domain

1-140aa

UniProt No.

P37840

NCBI Accession No.

NP_000336.1

Alternative names

SNCA, NACP, PARK1, PARK4, PD1, α-synuclein Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease 4, autosomal dominant Lewy body

Product Specification

Molecular Weight

14.4 kDa (140aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 0.1 M NaCl, 1mM MgCl2

Purity

> 95% by SDS-PAGE

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Tag

Non-Tagged

Applications

SDS-PAGE

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

Magnify

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Related Publications

Sung JY, et al. Proteolytic cleavage of extracellular secreted alpha-synuclein via matrix metalloproteinases. (J Biol Chem. 2005)
Ahn KJ, et al. Amino acid sequence motifs and mechanistic features of the membrane translocation of alpha-synuclein. (J Neurochem. 2006)
Kim EJ, et al. Dyrk1A phosphorylates alpha-synuclein and enhances intracellular inclusion formation. (J Biol Chem. 2006)
Lee HJ, et al. Assembly-dependent endocytosis and clearance of extracellular alpha-synuclein. (Int J Biochem Cell Biol. 2008)
Lee HJ, et al. Clearance and deposition of extracellular alpha-synuclein aggregates in microglia. (Biochem Biophys Res Commun. 2008)
Park JY, et al. Microglial phagocytosis is enhanced by monomeric alpha-synuclein, not aggregated alpha-synuclein: implications for Parkinsons disease. (Glia. 2008)
Lee HJ, et al. Direct transfer of alpha-synuclein from neuron to astroglia causes inflammatory responses in synucleinopathies. (J Biol Chem. 2010)
Kim KS, et al. Regulation of Weibel-Palade body exocytosis by alpha-synuclein in endothelial cells. (J Biol Chem. 2010)
Lee HJ, et al. Enzyme-linked immunosorbent assays for α-synuclein with species and multimeric state specificities. (J Neurosci Methods. 2011)
Kawakami F, et al. Stimulatory effect of α-synuclein on the tau-phosphorylation by GSK-3β. (FEBS J. 2011)
Kim KS, et al. Proteolytic cleavage of extracellular α-synuclein by plasmin: implications for Parkinson disease. (J Biol Chem. 2012)
Rodriguez-Araujo G, et al. Alpha-synuclein elicits glucose uptake and utilization in adipocytes through the Gab1/PI3K/Akt transduction pathway. (Cell Mol Life Sci. 2013)
Kim JY, et al. Nanomolar concentration of alpha-synuclein enhances dopaminergic neuronal survival via Akt pathway. (Neural Regen Res. 2013)
Kim HL, et al. The Presence of Outer Arm Fucose Residues on the N-Glycans of Tissue Inhibitor of Metalloproteinases-1 Reduces Its Activity. (J Proteome Res. 2014)
Rodriguez-Araujo G, et al. Low alpha-synuclein levels in the blood are associated with insulin resistance. (Sci Rep. 2015)
Takami Y, et al. Novel pathophysiological roles of α-synuclein in age-related vascular endothelial dysfunction. (FASEB J. 2022)