Product Information
- Product Type
- Cell Lysate
Product Specification
- Concentration
- 2mg/ml (determined by BCA assay)
- Formulation
- Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
- Storage
- Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
- Preparation before usage
- Heat lysate to 95C for 5 minutes and rapidly cooling before use.
- General Description
- NIH/3T3 cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition.
- Quality Control
- Western Blot analyzed by both GAPDH and Beta tubulin antibody
Data
Western blot analysis (WB)
All lanes : NIH/3T3 Whole Cell Lysate (40ug)
Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)
Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.