HeLa Lysate

Cat No.
ATGL0001

Size

QTY

List Price

100ug

USD 80


200ug

USD 110

500ug

USD 200

USD 0



Product Information

Product Type
Cell Lysate

Product Specification

Concentration
2mg/ml (determined by BCA assay)
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Storage
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Preparation before usage
Heat lysate to 95C for 5 minutes and rapidly cooling before use.
General Description
HeLa cells are human epithelial cells from a fatal cervical cancer cells taken from Henrietta Lacks, in 1951. The cell line was found to be remarkably durable and prolific as illustrated by its contamination of many other cell lines used in research. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes.
Quality Control
Western Blot analyzed by both GAPDH and Beta tubulin antibody

Data

Western blot analysis (WB)

All lanes : HeLa Whole Cell Lysate (40ug)

Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.