NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT1B2

UniProt No.

Q15388

NCBI Accession No.

NP_055580

Alternative names

Translocase of outer mitochondrial membrane 20, Translocase of outer mitochondrial membrane 20 homolog, Translocase of outer mitochondrial membrane 20 homolog type II, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOM20, MOM19, MAS20

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human Tomm20 (25-145aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF, FACS

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of Tomm20 in HeLa cells. The cell was stained with ATGA0482 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tomm20 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: HepG2 cell lysate
Lane 3.: A431 cell lysate
Lane 4.: K562 cell lysate
Lane 5.: A549 cell lysate
Lane 6.: 293T cell lysate
Lane 7.: MCF7 cell lysate
Lane 8.: SK-OV-3 cell lysate
Lane 9.: PC3 cell lysate

Flow cytometry (FACS)

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Flow cytometry analysis of Tomm20 in HeLa cells. The cell was stained with ATGA0482 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).