Human EEF1A1 antibody

Cat No.
ATGA0347

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT23C11
UniProt No.
P68104
NCBI Accession No.
NP_001393
Alternative names
Eukaryotic translation elongation factor 1 alpha 1, Leukocyte receptor cluster (LRC) member 7, Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, EEF1A, EF1A, LENG7, EE1A1, EF1A1, EF1alpha1

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human EEF1A1 (1-462aa) purified from E.coli
Isotype
IgG2a kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

ICC/IF analysis of EEF1A1 in A549 cells. The cell was stained with ATGA0347 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EEF1A1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: A549 cell lysate
Lane 3.: Raji cell lysate
Lane 4.: THP-1 cell lysate
Lane 5.: MCF-7 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.