NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

J1D2

UniProt No.

Q13158

NCBI Accession No.

NP_003815

Alternative names

Fas-associated via death domain, GIG3, MORT1, MGC8528, Fas-associated via death domain, FADD, Fas-associated via death domain FADD protein, Fas TNFRSF6 associated via death domain, Fas (TNFRSF6) associated via death domain, Fas associated via death domain, Fas associating protein, Fas associating death domain containing protein, Fas associating protein with death domain GIG 3, Growth inhibiting gene 3 protein, H sapiens mRNA for mediator of receptor induced toxicity, Mediator of receptor induced toxicity, MORT 1

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human FADD (1-208aa) purified from E. coli

Isotype

IgG2b kappa

Purification

By protein-G affinity chromatography

Applications

ELISA,WB,ICC/IF,IHC

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of FADD in HeLa cells. The cell was stained with AFA0901 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The cell lysates of Jurkat (30ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FADD (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FADD antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : Raw264.7 cell lysate
Lane 3. : MCF7 cell lysate
Lane 4. : A431 cell lysate
Lane 5. : Ramos cell lysate
Lane 6. : Raji cell lysate
Lane 7. : Balb/3T3 cell lysate

Immunohistochemistry (IHC)

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Paraffin embedded sections of human breast cancer tissue were incubated with anti-human FADD (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)