Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- 5E2
- UniProt No.
- Q86YL7
- NCBI Accession No.
- NP_006465
- Alternative names
- TIA2, T1A, T1 ALPHA GENE, T1 alpha, Podoplanin, PDPN, PA2.26 antigen, OTTHuMP00000044504, OTTHuMP00000009640, OTS8, Lung type I cell membrane-associated glycoprotein isoform a, Lung type I cell membrane associated glycoprotein isoform a AGGRuS, Lung type I cell membrane associated glycoprotein, hT1alpha2, hT1alpha1, HT1A-1, GP40, Gp38, GP36, Glycoprotein 36 KD
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human PDPN (1-206aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, ICC/IF, FACS
- Usage
- The antibody has been tested by ELISA, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of Podoplanin in HaLe cells. The cell was stained with APD0833 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow cytometry (FACS)
Flow cytometry analysis of Podoplanin in HeLa cells. The cell was stained with APD0833 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.