NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

k1C7

UniProt No.

P25685

NCBI Accession No.

NM_006145

Alternative names

Heat shock 40 kDa protein 1, DNAJB1, DNAJ1, HDJ1, HSPF1, HSP40 (DNAJ), Heat shock 40 kDa protein 1, Heat shock protein 40, HSP40, DnaJ protein homolog 1, HDJ-1, DnaJ homolog subfamily B member 1, Heat shock 40 kDa protein 1, radial spoke 16 homolog B (Chlamydomonas), Hsp40, Sis1, RSPH16B,

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human Hsp40(1-340aa) purified from E. coli

Isotype

IgG2a kappa

Purification

By protein-G affinity chromatography

Applications

ELISA,WB

Usage

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

Magnify

The Cell lysates of Jurkat (50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Magnify

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: HSP40 Transfected 293T cell lysate