NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

4E4

UniProt No.

P14174

NCBI Accession No.

NP_002406

Alternative names

Macrophage migration inhibitory factor, GLIF, MMIF, MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor, macrophage migration inhibitory factor (glycosylation-inhibiting factor),

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human MIF (1-115 aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of MIF in Balb/3T3 cells. The cell was stained with AMF0608 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

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ICC/IF analysis of MIF in HeLa cells. The cell was stained with AMF0608 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate
Lane 2.: THP-1 cell lysate
Lane 3.: HeLa cell lysate
Lane 4.: U937 cell lysate

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The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Related Publications

Koizumi M, et al. Positive correlations of age and parity with plasma concentration of macrophage migration inhibitory factor in Japanese black cows. (J Reprod Dev. 2016)