NKMAX

Product Information

Product Type

Recombinant Protein

Expression System

HEK293

Domain

29-766aa

UniProt No.

P27487

NCBI Accession No.

NP_001926.2

Alternative names

Dipeptidyl peptidase 4, dipeptidyl peptidase 4 isoform 1, ADABP, ADCP2, CD26, DPPIV, TP103, Adenosine deaminase complexing protein 2, Dipeptidyl peptidase IV, DPP4, T-cell activation antigen CD26

Product Specification

Molecular Weight

112.1kDa (977aa)

Concentration

0.25mg/ml (determined by Absorbance at 280nm)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Purity

> 95% by SDS-PAGE

Bioactivity

1.Measured by its binding ability in a functional ELISA with MERS-CoV Spike S1 Subunit (CAT# ATGP3980). The ED50 range ≤250 ng/ml.
2.Measured by its binding ability in a functional ELISA with MERS-CoV Spike RBD (CAT# ATGP4111). The ED50 range ≤200 ng/ml.
3.Measured by its binding ability in a functional ELISA with MERS-CoV Spike (CAT# ATGP4112). The ED50 range ≤120 ng/ml.
4.Specific activity is > 15,000 pmol/min/ug in which one unit defined as the amount of enzyme that hydrolyze 1pmole of H-Gly-Pro-AMC.HBr to H-Gly-Pro and AMC per minute at pH 8.0 at 37℃.

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Tag

hIgG-Tag

Applications

SDS-PAGE, Enzyme Activity, Bioactivity

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

Magnify

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Biological Activity

Magnify

MERS-CoV Spike S1 Subunit (CAT# ATGP3980) is coated at 2 ug/ml (100 ul/well) can bind IgK-DPP4. The ED50 range ≤250 ng/ml.

Magnify

MERS-CoV Spike RBD (CAT# ATGP4111) is coated at 2 ug/ml (100 ul/well) can bind IgK-DPP4. The ED50 range ≤200 ng/ml.

Magnify

MERS-CoV Spike (CAT# ATGP4112) is coated at 2 ug/ml (100 ul/well) can bind IgK-DPP4. The ED50 range ≤120 ng/ml.