Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 26-189aa
- UniProt No.
- P21583
- NCBI Accession No.
- NP_000890
- Alternative names
- Kit ligand, KITLG, Mast cell growth factor, MGF, Stem cell factor, SCF, c-Kit ligand, steel factor, SF, Kitl, KL-1, Familial progressive hyperpigmentation 2, FPH2, SLF, DFNA69
Product Specification
- Molecular Weight
- 18.5 kDa (165aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4)
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤10ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Human SCF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤10 ng/ml.
Related Publications
-
Lee SH, et al. Involvement of MITF-A, an alternative isoform of mi transcription factor, on the expression of tryptase gene in human mast cells. (Exp Mol Med. 2010)
Takahashi M, et al. CD133 is a positive marker for a distinct class of primitive human cord blood-derived CD34-negative hematopoietic stem cells. (Leukemia. 2014)
Yamada T, et al. CXCL12 regulates differentiation of human immature melanocyte precursors as well as their migration. (Arch Dermatol Res. 2019)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.