Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-165aa
- UniProt No.
- P62937
- NCBI Accession No.
- NP_066953.1
- Alternative names
- Peptidylprolyl isomerase A, CYPA, CYPH, MGC12404, MGC23397, MGC117158, Cyclophilin A, Cyclosporin A binding protein, Peptidyl prolyl cis trans isomerase A, PPIA, PPIase A, Rotamase A, T cell cyclophilin
Product Specification
- Molecular Weight
- 20kDa (185aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20mM NaCl, 0.5mM DTT, 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 650nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Related Publications
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Gallay PA, et al. The Novel Cyclophilin Inhibitor CPI-431-32 Concurrently Blocks HCV and HIV-1 Infections via a Similar Mechanism of Action. (PLoS One. 2015)
Gallay PA, et al. Characterization of the Anti-HCV Activities of the New Cyclophilin Inhibitor STG-175. (PLoS One. 2016)
Kim K, et al. Recombinant human Cyclophilin A stimulates hair follicle cells via Wnt/β-catenin signaling pathway. (Biotechnol Lett. 2019)
Tsuda T, et al. Rho-associated protein kinase and cyclophilin a are involved in inorganic phosphate-induced calcification signaling in vascular smooth muscle cells. (J Pharmacol Sci. 2020)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.